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. 1996 Nov 26;93(24):13635–13640. doi: 10.1073/pnas.93.24.13635

Figure 1.

Figure 1

Iron EPR signals from whole-cell preparations. EPR parameters were: T = −125°C, field center = 1520 G, field sweep = 500 G, modulation amplitude = 25 G, receiver gain = 32,000, frequency = 9.006 GHz, and power = 30 mW. One thousand data points were collected per scan, and four scans were averaged per sample. Scan profiles for samples B–F have been normalized to cell concentration. Scans: A, iron standard; B, AB1157 (wild type), 10 μM intracellular chelatable iron; C, KK204 (Fur−), 70 μM iron; D, JI132 (SOD−), 80 μM iron; E, KK216 (SOD− Fur−), 160 μM iron; F, same AB1157 scan as B with 13-fold amplification of signal.