Table 1.
Strains and plasmids used in this study
| Strain or plasmid | Genotype | Source or ref. |
|---|---|---|
| Strains | ||
| AB1157 | F−thr-1 leuB6 proA2 his-4 thi-1 argE2 lacY1 galK2 | 14 |
| rpsL supE44 ara-14 xyl-15 mtl-1 tsx-33 | ||
| JI132 | As AB1157 plus (sodA::Mud PR13)25(sodB::kan)1-Δ2 | 14 |
| BN407 | As A1157 plus ΔlacU169 and ColV-K30 (iucC::lacZ) | 30 |
| HB351 | F− Δ(argF-lac)169 Δ(edd-zwf)22 zeb-1::Tn10 | 31 |
| JRG2953 | As W3110 plus bfr::kan ftn::spec | John Guest |
| KK204 | As AB1157 plus fur::Tn5 | 8 |
| KK206 | As AB1157 plus bfr::kan ftn::spec | P1(JRG2953) × AB1157 |
| KK209 | As JI132 plus bfr::kan ftn::spec | P1(JRG2953) × JI132 |
| KK216 | As JI132 plus fur::Tn5 zbf-507::Tn10 | 8 |
| KK222 | As JI132 plus Δ(edd-zwf)22 zeb-1::Tn10 | P1(HB351) × JI132 |
| KK226 | As AB1157 plus Δ(edd-zwf)22 zeb-1::Tn10 | P1(HB351) × AB1157 |
| KK230 | As KK226 plus pKK3 | This study |
| KK231 | As KK222 plus pKK3 | This study |
| KK260 | As KK226 plus pCKR101 | This study |
| KK261 | As BN407 plus pKK3 | This study |
| KK266 | As KK222 plus pKK3 | This study |
| Plasmids | ||
| pBN44 | pUC18 derivative containing zwf-eda | 32 |
| pCKR101 | pBR328-derived vector containing lacIq and Ptac | Jeff Gardner |
| pKK3 | pCKR101 containing zwf-eda | This study |