TABLE 1.
Time (h) | Sample no.a | Glycerol concn (mM) | 1,3-PDL concn (mM) | Growth (OD)b |
---|---|---|---|---|
0 | 1 | 0.00 | 0.41 | 0.1 |
2 | 37.78 | 0.96 | 0.09 | |
3 | 36.7 | 0.82 | 0.09 | |
24 | 1 | 0.00 | 0.55 | 3.85 |
2 | 19.11 | 15.32 | 2.79 | |
3 | 19.54 | 16.68 | 2.98 |
Sample numbers correspond to growth and fermentation of samples in B12-free medium. Sample 1, without glycerol; sample 2, with glycerol; sample 3, with glycerol and 20 nM cyanocobalamin.
OD, optical density at 560 nm.