Table 2.
Summary of strand-displacement experiments
| Reaction sequence |
|---|
| Efficient strand displacement |
| Cy3-TAR/Cy5-zipper DNA + Cy5-cTAR |
| Cy3-TAR/Cy5-loop DNA + Cy5-cTAR |
| Cy3-TAR/Cy5-zipper RNA + Cy5-cTAR |
| Cy3-TAR/Cy5-loop RNA + Cy5-cTAR |
| Cy3-TAR/Cy5–14-mer DNA + cTAR |
| Inefficient strand displacement |
| Cy3-TAR/cTAR + Cy5-cTAR |
| Cy3-TAR/cTAR + Cy5-loop DNA |
| Cy3-TAR/cTAR + Cy5-cTAR + Cy5–14-mer DNA |
The annealing and replacement experiments were run in buffer containing 0.2 mM Mg2+ and 889 nM NC, 25 nM Cy5-zipper/loop nucleotide, and Cy5-cTAR DNA with the exception that the Cy5–14-mer DNA measurement was run with 50 nM Cy5–14-mer DNA and 2.5 nM unlabeled cTAR.