Coinjection strategy to search for a distal regulatory region(s). (a) The 1.5-kb upstream region of RH2–1 conjugated to the GFP reporter. (b) Restriction fragments used for the coinjections. The 10-kb BamHI (B/B) fragment capable of inducing GFP expression is depicted as a hatched bar. (c) Restriction fragments from the 10-kb BamHI region used for the coinjection. The fragments capable of inducing GFP expression are depicted as hatched bars. B, BamHI; Bg, BglII; E, EcoRI; K, KpnI; Sc, SacI; S, SalI.