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Effect of RH2-LCR elimination from the RH2/GFP-PAC clones on GFP expression in SDCs. (a) Construction of the RH2/GFP-PACΔLCR clones from the RH2/GFP-PAC clones. The RH2-LCR was replaced with the Kmr gene flanked on both sides by the FRT sequences through homologous recombination; the Kmr and one FRT were removed by flpe induction. (b) Expression levels of the GFP reporter in zebrafish injected with the RH2/GFP-PACΔLCR clones. The histogram is shown as in Fig. 1b.
