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. 2007 Jul 23;104(31):12813–12818. doi: 10.1073/pnas.0704061104

Fig. 5.

Fig. 5.

Positional effect of the RH2-LCR in the RH2/GFP-PAC clones on GFP expression in SDCs. (a) Distances from the RH2-LCR to the four RH2 opsin genes in the RH2-PAC clone. (b) Construction of RH2/GFP-PAC>LCR clones from the RH2/GFP-PACΔLCR clones. The RH2-LCR, coupled with Kmr and one FRT sequence, was inserted into the position immediately downstream of RH2–3 in RH2/GFP-PACΔLCR clones by homologous recombination. The Kmr-FRT region is depicted as an open box after the recombination. Distances from the RH2-LCR to the four RH2 opsin genes in the RH2/GFP-PAC>LCR clones are shown. (c) Expression levels of the GFP reporter in zebrafish injected with the RH2/GFP-PAC>LCR clones. The histogram is shown as in Fig. 1b.