TABLE 5.

Gradient plate analysis of abilities of chimeric MFPs to support AcrB-catalyzed drug efflux in HNCE3 cells

Construct	Length of growth zone (mm)a in the presence ofb:
	Cholic acid	Taurocholic acid	Fusidic acid	Novobiocin	Crystal violet	Ethidium bromide	Ciprofloxacin	Chloramphenicol	Erythromycin	Tetracycline
pSportI	37	30	10	19	16	33	43	9	35	20
pAcrA	80	80	80	80	80	80	80	80	80	80
pYhiU	36	38	8	19	17	30	47	5	32	14
pAuN66	80	80	80	80	80	80	80	80	80	80
pAuN210	80	80	80	80	80	80	80 (38)c	80	80	80
pAuN290	80	80	80	80	80	80	80 (35)	68	80	80
pAuN357	35	38	10	13	10	36	32	15	35	7
pAuC210	37	30	14	17	15	35	43	7	32	8
pAuC290	40	35	13	17	14	40	40	10	35	11
pAuC357	80	80	80	80	80	80	80	80	80	80

^aSee footnote a of Table 2. Values shown in boldface represent constructs that activated presumably AcrB-driven drug efflux.

^bSee footnote b of Table 4.

^cNumbers in parentheses indicate the length of heavy growth seen.