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Tumor recognition by vaccine-induced CD4+ T cells. (A) ESO157–170-specific CD4+ T cells were assessed for recognition of ESO+DP4+ SK-Mel-37 or ESO−veDP4+ SK-Mel-23 by intracellular TNF-α staining. (B) Reactivity of CD4+ T cell clones to ESO157–170 peptide with concentration ranging from 10 μM to 1 nM. (C) Only clone 5-B-8 recognized SK-Mel-37, and SK-Mel-23 was only recognized by all clones when pulsed with ESO157–170. (D and E) Assessment of tumor recognition by IFN-γ ICS.
