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CD8+ T cell response to ESO157–170 vaccine. (A) CD8+ T cells were stimulated with ESO157–170 peptide and stained with HLA-A2/ESO157–165 multimer or HLA-A2/Flu HA58–66 multimer. (B) IFN-γ production by ESO157–170 specific CD8+ T cells was analyzed by ELISPOT. (C and D) Confirmation by IFN-γ ICS.
