Fig. 5.

Tumor recognition by vaccine-induced CD8⁺ T cells. (A) After vaccine, ESO_157–170-specific CD8⁺ T cells from patient 18 were tested against ESO-1b or irrelevant peptide Flu-HA in a CD107a/b surface and IFN-γ ICS assay. (B) HLA-A2/ESO_157–165 multimer-reactive CD8⁺ T cells recognized HLA2⁺ESO⁺ cell line MZ-MEL-19 but not HLA-A2⁺ESO⁻ line SK-MEL-23. (C) Reactivity of CD8⁺ T cell clones to ESO_157–165 peptide (range 10 nM to 1 pM). (D–F) All four CD8⁺ T cell clones recognized HLA2⁺ESO⁺ MZ-Mel-19 but not HLA2⁺ESO⁻ SK-Mel-23.