Table 1.
The effect of FXI inhibition on the activation of the coagulation cascade in plasma
| CTI added to blood draw tubes |
CTI added to PPP after plasma preparation | |||||
|---|---|---|---|---|---|---|
| PRP | PPP | |||||
| FXI antibody | - | + | - | + | - | + |
| Recalcification | 65.1 ± 4.5 | 71.0 ± 12.5 | 90 ± 0 | 90 ± 0 | 15.5 ± 2.3 | 25.5 ± 5.6* |
| TF 10−6 | 37.1 ± 6.8 | 34.4 ± 4.2 | 81.8 ± 14.2 | 86.6 ± 5.8 | 14.5 ± 2.6 | 20.6 ± 3.9* |
| TF 2.5 × 10−6 | 24.9 ± 1.9 | 25.3 ± 1.4 | 66.9 ± 15.4 | 68.8 ± 19.7 | 13.2 ± 2.2 | 17.9 ± 3.3* |
| TF 1.25 × 10−5 | 13.4 ± 0.5 | 15.1 ± 1.8 | 19.5 ± 4.2 | 19.3 ± 3.9 | 10.1 ± 1.5 | 12.2 ± 1.9* |
| Thrombin 50 pM | 24.1 ± 6.7 | 23.2 ± 5.3 | 58.6 ± 4.8 | 68.0 ± 3.9 | 10.8 ± 0.2 | 13.9 ± 0.5* |
| Thrombin 150 pM | 22.3 ± 3.7 | 21.0 ± 4.0 | 37.8 ± 4.0 | 41.9 ± 1.7 | 8.6 ± 0.7 | 10.8 ± 0.9* |
PRP or PPP from three healthy donors prepared from blood drawn into citrate or citrate + CTI was preincubated with 10 μg/ml of FXI antibody 5061 or mouse IgG and CTI (when it was not included in the drawing tubes). After preincubation, plasma was stimulated with the indicated procoagulant. The average time (in min) for the three donors is shown. Conditions where the P value of a paired t test for reactions with or without the FXI antibody is ≤ 0.05 are denoted with *.