Skip to main content
. 2007 Jul 23;104(31):12861–12866. doi: 10.1073/pnas.0702509104

Fig. 3.

Fig. 3.

Hepatic SIRT1 controls systemic and hepatic cholesterol and fatty acid homeostasis. (A) Liver free fatty acids (nonesterified fatty acids), serum total cholesterol, and liver cholesterol from control shRNA-infected fed (n = 5) and 20-h fasted (n = 5) mice and SIRT1 shRNA-infected fed (n = 6) and fasted (n = 5) mice. (B) Fed, GFP (n = 6) and SIRT1 (n = 6); 19-h fasted, GFP (n = 10) and SIRT1 (n = 10). (C) GFP or SIRT1 overexpression and control or PGC-1α shRNA double-infected mice (each bar, n = 5) following a 20-h fast. Liver measurements were normalized to protein content. Similar results for all measurements have been observed in at least two independent experiments. All data are presented as the average ± SEM. Significance was determined by Student's t test. GFP/control shRNA vs. SIRT1/SIRT1 shRNA: *, P < 0.05; **, P < 0.01. Control shRNA vs. PGC-1α shRNA: #, P < 0.05.