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. 2007 Jul 23;104(31):12902–12906. doi: 10.1073/pnas.0702500104

Fig. 4.

Fig. 4.

Quantitation of mycovirus RNA in dicer mutant C. parasitica strains. (A) Agarose gel analysis of relative dsRNA accumulation for hypovirus CHV1-EP713 and mutant strain Δp29, which lacks suppressor of RNA silencing p29, in wild-type strain EP155 and the Δdcl-2 deletion mutant strain. Lane M, 1-kb DNA ladder size markers; lane 1, CHV1-EP713-infected control strain EP155; lane 2, Δp29-infected strain EP155; lane 3, Δp29-infected Δdcl-2 mutant strain. The migration positions of hypovirus dsRNAs are shown at the right. The slowest migrating band represents full-length viral dsRNA, whereas the faster migrating bands represent internally deleted viral dsRNAs commonly generated by hypoviruses (47). The migration positions of rRNAs are indicated by asterisks. Results of semiquantitative RT-PCR analysis of total viral RNA in the corresponding CHV1-EP713 and Δp29-infected strains relative to 18S rRNA sequences are shown in the chart below the agarose gel. The values are normalized to the viral RNA accumulation in Δp29-infected strain EP155 (set to a value of 1), with the standard deviation based on three independent measurements of two independent RNA preparations indicated by the error bars. (B) Agarose gel analysis of viral dsRNA accumulation in reovirus MyRV1-Cp9B21-infected strain EP155 (lane 1), infected mutant Δdcl-1 (lane 2), infected mutant Δdcl-1 (lane 3), and infected double mutant Δdcl-1/Δdcl-2 (lane 4). Lane M contains 1-kb DNA ladder size markers. The migration position of the three largest MyRV1-Cp9B21 dsRNA segments are shown at the right. Results of semiquantitative RT-PCR analysis of MyRV1-Cp9B21 RNA in the corresponding strains are shown in the chart below the agarose gel. In this case, the relative amount of total viral RNA was estimated by measuring the amount of segment S3-specific RNA relative to 18S rRNA (42). Values were normalized to the amount of S3-specific RNA in strain EP155 (set to a value of 1), with the standard deviation based on three independent measurements of two independent RNA preparations indicated by the error bars.