TABLE 3.
Substrate | EaCoMT activity (nmol/min/mg of protein)a,b (n)
|
|
---|---|---|
mc2155(pMV-CoM) | mc2155(pMV261) | |
Epoxyethane | 615 ± 98 (6) | 12 ± 20 (3) |
Epoxypropane | 143 ± 23 (4) | 1 ± 3 (4) |
Averages and standard deviations derived from several independent experiments.
Activities calculated from substrate depletion rate, after subtraction of abiotic rates of substrate loss in controls without cell extract. The abiotic rates of epoxyethane and epoxypropane loss were 13 ± 2 and 12 ± 2 nmol/min, respectively.