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. 2003 Sep;185(18):5465–5472. doi: 10.1128/JB.185.18.5465-5472.2003

FIG. 3.

FIG. 3.

Detection by Western blot analysis of PBP2a (78 kDa) encoded by mecA. COLn(pAW8) is the positive control; COLnex(pAW8), RN4220(pAW8), and RN4220(pZRI)(pAW8) are the negative controls. (a) Comparison of experienced host and naive host transformants carrying pYK20. (b) Representative transformants with cloning vector pAW8 only, pYK644 (carrying intact mecR1-mecI-mecA), and pYK20 (carrying unregulated mecA) and cotransformants with pZRI (carrying blaR1-blaI-blaZ). All were grown under noninducing conditions.