TABLE 2.
Synthetic oligonucleotide primers
| Genetic elementa and primer designation | Sequenceb | Nucleotide positions |
|---|---|---|
| mecA | ||
| K15 | 5′-AGCACACCTTCATATGACGTCT-3′ | 32473-32494 |
| K16 | 5′-TGGATCAAAATTGGGTACAAGA-3′ | 31991-32012 |
| K17 | 5′-AGTTGTAGTTGTCGGGTTTGGT-3′ | 31425-31446 |
| K18 | 5′-TGGCAATATTAACGCACCTCACT-3′ | 33012-33034 |
| K34 | 5′-TATGCGGATCCTCGTGTCAGATACATTTCGATTCA-3′ | 31068-31091 |
| K38 | 5′-ATTTCGGATCCGTTGTAGCAGGAACACAAATGAATAAC-3′ | 33645-33619 |
| lacZ | ||
| K3 | 5′-TATGTTGTGTGGAATTGTGAGCGGA-3′ | 167-191 |
| K4 | 5′-AGGCGATTAAGTTGGGTAACGCCAG-3′ | 329-353 |
| blaR1-blaI, P1-101 | 5′-GTCGATAAGCTTTTAATTCAGCACTAAACTTTTCATG-3′ | 2205-2184 |
a
The primers listed are specific for mecA genes of strain NCTC 10442 (GenBank accession no. AB033763), for lacZ in pUC119 (GenBank accession no. U07649), and for blaR1-blaI in pI258 (GenBank accession no. M62650).
b
Underlining indicates BamHI sites introduced in K34 and K38 and HindIII site introduced in P1-101.