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Detection by PCR of mecA insert in plasmid pYK20
| Transformant | No. of transformants | Fragment ratioa | Relative amt of PBP2 (%)b |
|---|---|---|---|
| COLnex(pYK20) | 125 | 0 | 96 |
| RN4220(pYK20) | 100 | 24.4 | 33 |
| 8325-4(pYK20) | 53 | 4.4 | 107 |
| 1-63(pYK20) | 26 | 70 | 18 |
Ratio of unexpected <2.8-kb fragments in to total fragments screened transformants.
Percent density of PBP2a in each transformant compared to PBP2a in COLn.
