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Footprint analysis of the FurA binding site upstream of M. tuberculosis furA. The purified −154/+33 DNA region, labeled at either the +33 end (A) or the −154 end (B), was incubated in the presence of the amount of FurA protein (in micrograms) indicated above the lanes for 20 min at room temperature and digested with DNase I as described in Materials and Methods. Maxam-Gilbert A+G sequences of the same fragments were loaded in the first lanes.
