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Effect of mutations in the furA operator sequence on FurA binding. (A) Sequences of the mutations. Fragment A, wild type. Fragment B, C, and D carry different 6-bp substitutions within the AT-rich region (boxed). The mutant bases are indicated by asterisks. The −10 and −35 sequences are in boldface. (B) Gel shift of the mutant fragments caused by FurA. FurA binding was performed as described in the legend to Fig. 4.
