TABLE 1.
Luciferase activity expressed by mutant DNA fragments.
| Plasmida | Luciferase RLU (103)b
|
|
|---|---|---|
| Without H2O2 | With H2O2 | |
| pMYT101 | 869 ± 176 | 1,080 ± 202 |
| pMYT102 | 1,682 ± 370 | 1,638 ± 332 |
| pMYT103 | 9,528 ± 1,054 | 8,549 ± 1,314 |
| pMYT104 | 9,632 ± 2,566 | 9,616 ± 2,844 |
a
The plasmids were derived from pSG10ter by insertion of the M. tuberculosis −154/+33 fragments upstream of the luxAB reporter gene. pMYT101 carries the wild-type sequence, and pMYT102, pMYT103, and pMYT104 carry the B, C, and D mutant sequences indicated in Fig. 6, respectively.
b
Luciferase activity was measured as described in the legend to Fig. 1. The cultures were treated with 0.062 mM H2O2 for 1 h. The mean values ± standard deviations for four independent clones tested are reported.