TABLE 2.

Sporulation frequencies of wild-type and mutant M. xanthus strains

Strain	Genotype	Sporulation frequencya (h)
		48	72	96	120
DK1622	Wild type	<0.0006	0.7 ± 0.2	12 ± 4	100 ± 16
SA1634	todK::mini-Tn5(tet) Ω8846	<0.0006	13.3 ± 3.9	78 ± 16	278 ± 79
SA1681	ΔtodK	<0.0006	21.9 ± 5.1	122 ± 16	305 ± 39
SA1682	ΔdotR	<0.0006	1.4 ± 0.4	15 ± 0.4	139 ± 39
SA1686	todK::mini-Tn5(tet) Ω8846 attB::pSWU19	<0.0006	18.3 ± 6.3	92 ± 20	278 ± 79
SA1688	todK::mini-Tn5(tet) Ω8846 attB::pAAR136	<0.0006	2.5 ± 1.2	31 ± 4	150 ± 24

^aCells were starved on CF starvation medium for the indicated periods of time. Sporulation frequencies are presented relative to the level of sporulation in DK1622 after 120 h as mean values and standard deviations from four experiments. Spore titers were determined as the numbers of sonication- and heat-resistant CFU (58). The absolute sporulation frequency of DK1622 after 120 h was 4.0%.