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Primers used in PCR exprimentsa
| Primer no. | Sequence | Gene |
|---|---|---|
| 1 | 5′-TTTCCCGAGCTCATGCAGCAGTTAGCCAGTTTC | maoCEc |
| 2 | 5′-GCTCTAGATTAATCGACAAAATCACCGTG | maoCEc |
| 3 | 5′-TTTCCCGAGCTCATGCAGCAGTTAGCCAGTTTC | maoCEc-His6 tag |
| 4 | 5′-GCTCTAGATTAATGGTGATGATGGTGATGATCGACAAAATCACCGTG | maoCEc-His6 tag |
| 5 | 5′-GGAATTCATGGAAATGACATCAGCGTTTACC | yfeXEc |
| 6 | 5′-CCCAAGCTTTTATTGCAGGTCAGTTGC | yfcXEc |
Restriction enzyme sites are shown in boldface type. The template for all primers was the E. coli W3110 chromosome.
