TABLE 3.

Results of flask cultures of recombinant E. coli strains harboring different sets of plasmids^a

Strain	Condition	DCW (g/liter)	PHA concn (g/liter)	PHA content (wt %)	Monomer composition (mol%)b
					3HB	3HHx	3HO	3HD
W3110	pMCS104613C2 + p10499A	1.50 ± 0.01	0	0	0	0	0	0
W3110	pMCS104613C2 + p10499B2341	1.20 ± 0.04	0	0	0	0	0	0
W3110	pMCS104613C2 + p10499MaoC	1.95 ± 0.02	0.23 ± 0.04	11.9 ± 2.0	0	19 ± 2	74 ± 2	7 ± 2
WB101	pMCS104613C2 + p10499A	0.97 ± 0.01	0.21 ± 0.01	21.0 ± 1.4	0	9 ± 2	37 ± 2	54 ± 2
WB101	pMCS104613C2 + p10499MaoC	1.00 ± 0.03	0.20 ± 0.01	20.0 ± 1.6	0	0	61 ± 2	39 ± 2
WB101	pMCS104613C2 + p10499B2341	1.13 ± 0.02	0.39 ± 0.05	34.5 ± 3.5	0	9 ± 2	33 ± 2	58 ± 2
WB112	pMCS104613C2 + p10499A	0.80 ± 0.01	0.05 ± 0.01	6.3 ± 1.1	0	8 ± 2	36 ± 2	56 ± 2
WB112	pMCS104613C2 + p10499B2341	1.15 ± 0.01	0.30 ± 0.02	26.0 ± 1.9	0	10 ± 2	34 ± 2	56 ± 2
WB108	pMCS104613C2 + p10499A	0.78 ± 0.01	0.12 ± 0.01	15.0 ± 1.8	0	9 ± 2	52 ± 2	37 ± 2
WB108	pMCS104613C2 + p10499MaoC	0.80 ± 0.01	0.16 ± 0.01	20.0 ± 1.3	0	0	61 ± 2	39 ± 2

^aCells were cultivated for 96 h at 30°C in LB medium supplemented with sodium decanoate (2 g/liter). All experiments were carried out in triplicate.

^bAbbreviations: 3HB, (R)-3-hydroxybutyrate; 3HHx, (R)-3-hydroxyhexanoate; 3HO, (R)-3-hydroxyoctanoate; 3HD, (R)-3-hydroxydecanoate.