Skip to main content
. 2003 Sep;185(18):5527–5535. doi: 10.1128/JB.185.18.5527-5535.2003

FIG. 3.

FIG. 3.

Immunoblots of soluble F1Fo (A) and F1 (B) from C. pasteurianum (Cp) and E. coli DH5α (Ec) after reactions with antibodies against purified F1Mt. (A) Soluble F1FoCp after precipitation with ammonium sulfate from DM extracts (1.0% [wt/vol]) of C. pasteurianum membranes (30 μg) and soluble F1FoEc in DM extracts (1.0% [wt/vol]) of E. coli membranes (30 μg). (B) Partially purified F1Cp (30 μg) and F1Ec (30 μg). Proteins were separated by native PAGE (7.5% acrylamide) and transblotted onto polyvinylidene difluoride membranes prior to immunoreactions.