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. 2003 Sep;185(18):5611–5626. doi: 10.1128/JB.185.18.5611-5626.2003

FIG. 6.

FIG. 6.

Aligned genome images of DNA microarrays of MG1655 (CGSC 6300) and prototrophic derivatives of clinical isolates of E. coli. The strains were MG1655 (CGSC 6300), rows 1 and 5; derivative of NCM3601 (a urinary tract isolate), row 2; derivative of NCM3610 (a urinary tract isolate), row 3; derivative of NCM3611 (an intestinal commensal isolate), row 4. They were grown in N−C− medium with 0.4% glucose as the carbon source and 5 mM glutamine or 10 mM NH4Cl as the nitrogen source. The cDNAs from cells grown with glutamine or NH4Cl were labeled with Cy5 (red) or Cy3 (green), respectively. Operons thought to be in the extended NtrC/Nac regulon (60) that are more highly expressed on glutamine in all strains include glnK-amtB (b0450 to b0451), glnHPQ (b0809 to b0811), potFGHI (b0854 to b0857), ycdGHIJKLM (b1006 to b1012), ydcSTUVW (b1440 to b1444), ddpXABCDE (b1483 to b1488), yeaGH (b1783 to b1784), yedL (b1932), cbl (b1987), nac (b1988), argT (b2310), ygjG (b3073), and glnALG (b3868 to b3870). See text for discussion of other differences in expression. A different print was used for the experiment in row 5 than for the others.