TABLE 6.
Doubling times on galactose of MG1655 (CGSC 6300) derivatives carrying defined nag lesionsa
| Strain | Relevant genotype | Doubling time (min) |
|---|---|---|
| MG1655 | Wild type | 280 |
| NCM3789 | nagE::Kanr | 300 |
| NCM3817 | nagB::Kanr | 85 |
| NCM3818 | nagB2 | 240 |
| NCM3786 | nagA::Tetr | 80 |
| NCM3787 | nagC::Tetr | 85 |
| NCM3821 | nagD::Tetr | 260 |
| NCM3820 | Δnag(EBACD)::Tetr | 80 |
| NCM3851 | nagA::TetrnagE::Kanr | 85 |
| NCM3852 | nagC::TetrnagE::Kanr | 80 |
| NCM3105/pBR322 | Wild type | 270 |
| NCM3105/pBRnagC+ | nagC+/nagC+ | 250 |
| NCM3787/pBR322 | nagC::Tetr | 85 |
| NCM3787/pBRnagC+ | nagC::Tetr/nagC+ | 155 |
| NCM3820/pBR322 | Δnag(EBACD)::Tetr | 80 |
| NCM3820/pBRnagC+ | Δnag(EBACD)::Tetr/nagC+ | 80 |
a
Precultures were grown on glucose. NCM3845 (nagA166), which was isolated from MG1655 as a galactose fast-grower, had the same doubling time as NCM3786 (nagA::Tetr) (75 min), whereas MG1655 had a doubling time of 240 min in this experiment.