FIG. 2.

Expression of Fosβ and Junβ in the E. coli strain UT5600. (A) Immunoblot of whole-cell protein extracts from induced cultures expressing Fosβ (lane 1) or Junβ (lane 2) or nontransformed (lane 3). The blot was probed with anti-E tag MAb. Streptavidin-peroxidase conjugate was also added to detect the biotinylated protein standards (M). (B) Accessibility of the peptidoglycan of the same induced E. coli cells. The signals of intact cells, using an antipeptidoglycan serum (38), are shown as a percentage of lysed cells. Nontransformed E. coli strain UT5600 was used as control.