TABLE 3.
sopE1 polymorphism among SPV and EPV strains of S. enterica serotype Paratyphi B and other serotypes and lysogens of S. enterica strains
| Strain | Serotype | Pathovar | sopE1 PCR result | sopE1 RFLP patterna |
sopE1 (I) PCR resultb
|
sopE1 (II) PCR resultc
|
||
|---|---|---|---|---|---|---|---|---|
| Upstream to orfR | Downstream to orf45 | Upstream to orfR | Downstream to orf194 | |||||
| Tester strains (lysogen) | ||||||||
| B309 | Paratyphi B | SPV | + | II | − | − | + | + |
| 00-08652 | Paratyphi B | EPV | − | − | − | − | − | − |
| 00-08652 (ΦSopE309)d | Paratyphi B | EPV | + | II | − | − | + | + |
| A36 | Typhimurium | − | − | − | − | − | − | − |
| A36 (SopEΦ)e | Typhimurium | − | + | I | + | −g | − | − |
| A36 (ΦSopE309)f | Typhimurium | − | + | II | − | − | + | + |
| Clinical strains | ||||||||
| 99-06072 | Paratyphi B | SPV | + | II | − | − | + | + |
| 99-02820 | Paratyphi B | SPV | + | II | − | − | + | + |
| 99-08309 | Paratyphi B | SPV | + | II | − | − | + | + |
| 99-06148 | Paratyphi B | SPV | + | II | − | − | + | + |
| 97-15877 | Paratyphi B | SPV | + | II | − | − | + | + |
| SAR41 | Paratyphi B | SPV | + | II | − | − | + | + |
| 99-08380 | Paratyphi B | SPV | + | I | − | − | + | + |
| 96-01098 | Paratyphi B | EPVh | + | I | + | − | − | + |
| 97-12134 | Paratyphi B | EPVh | + | I | + | + | − | − |
| 99-01096 | Paratyphi B | EPVh | + | I | + | + | − | − |
| 99-01097 | Paratyphi B | EPVh | + | I | + | + | − | − |
| 99-08163 | Paratyphi B | EPVh | + | I | − | − | − | − |
| 99-04814 | Paratyphi B | EPVh | + | I | − | + | + | − |
| 96-08640 | Typhi | − | + | I | + | + | − | − |
| RKI130 | Heidelberg | − | + | I | − | − | + | + |
| 98-11635 | Typhimurium DT68i | + | I | + | + | − | − | |
| 93-00080 | Typhimurium DT204i | + | I | + | + | − | − | |
| 75-01646 | Typhimurium DT175i | + | I | + | + | − | − | |
| 76-E8 | Enteritidis | + | II | − | − | + | + | |
| 63-00647 | Gallinarum | + | II | − | − | + | + | |
| 99-03279 | Hadar | + | II | − | − | + | + | |
| 2229 | Dublin | + | II | − | − | + | + | |
Cluster I sopE1 PCR (17).
Cluster II sopE1 PCR (17).
Lysogenization of 00-08652 (EPV phage-free tester strain) with ΦSopE309 (isolated from SPV S. enterica serotype Paratyphi B strain B 309).
Lysogenization of the S. enterica serotype Typhimurium phage-free tester strain A36 with the sopE1-carrying bacteriophage SopEΦ isolated from clinical S. enterica serotype Typhimurium strains (17).
Lysogenization of the S. enterica serotype Typhimurium phage-free tester strain A36 with the sopE1-carrying bacteriophage ΦSopE309 isolated from systemic pathovar of S. enterica serotype Paratyphi B strain B 309.
The PCR is negative because a kanamycin resistance gene cassette has been introduced (17).
Enteric pathovar carrying the sopE1 determinant.
DT, definitive phage type.