TABLE 2.
Primers used for amplification of DNA segments from katG, inhA, and oxyR-ahpC genesa
| Gene | Primer sequence | Primer name | Size of product (bp) | Primer coordinates |
|---|---|---|---|---|
| katG | 5′-CATGAACGACGTCGAAACAG-3′ | katG 1 | 232 | 2153887-2156109 |
| 5′-CGAGGAAACTGTTGTCCCAT-3′ | katG 2 | |||
| ahpC | 5′-GCCTGGGTGTTCGTCACTGGT-3′ | ahpC 1 | 359 | 2726191-2726778 |
| 5′-CGCAACGTCGACTGGCTCATA-3′ | ahpC 2 | |||
| inhA | 5′-GAACTCGACGTGCAAAAC-3′ | inhA 1 | 206 | 1674200-1675009 |
| 5′-CATCGAAGCATACGAATA-3′ | inhA 2 |
a
The primer coordinates refer to the positions in the M. tuberculosis H37Rv genome (GenBank accession number AL123456).