TABLE 2.
Oligonucleotide primers and probes
| Designation | Sequencea | Amplicon size (bp) | Target | Source or reference |
|---|---|---|---|---|
| Primers | ||||
| Smut3368-F | 5′-GCCTACAGCTCAGAGATGCTATTCT-3′ | 114 | gtfB | UA159 |
| Smut3481-R | 5′-GCCATACACCACTCATGAATTGA-3′ | |||
| Ssob287-F | 5′-TTCAAAGCCAAGACCAAGCTAGT-3′ | 88 | gtfT | OMZ176 |
| Ssob374-R | 5′-CCAGCCTGAGATTCAGCTTGT-3′ | |||
| Uni152-F | 5′-CGCTAGTAATCGTGGATCAGAATG-3′ | 69 | 16S rRNA | 21 |
| Uni220-R | 5′-TGTGACGGGCGGTGTGTA-3′ | |||
| Fluorescent probes | ||||
| Smut3423T | 5′-FAM-TGGAAATGACGGTCGCCGTTATGAA-TAMRA-3′ | gtfB | UA159 | |
| Ssob298T | 5′-FAM-CCTGCTCCAGCGACAAAGGCAGC-TAMRA-3′ | gtfT | OMZ176 | |
| Uni177T | 5′-FAM-CACGGTGAATACGTTCCCGGGC-TAMRA-3′ | 16S rRNA | 21 |