TABLE 2.
Comparison of Ct values in monoassay and multiplex assay after real-time amplification of a dilution series of an L. pneumophila serogroup 1 standard
CFU/ml | Ct
|
|||
---|---|---|---|---|
Monoassay
|
Multiplex assay
|
|||
L. pneumophila | Legionella spp. | L. pneumophila | Legionella spp. | |
100,000 | 27.2 | 27.7 | 27.4 | 27.8 |
10,000 | 31.2 | 30.6 | 31.6 | 32.8 |
1,000 | 34.3 | 34.5 | 35.2 | 35.5 |
100 | 38.5 | 38.3 | 38.6 | 39.4 |
10 | 43.4 | 42.4 | 43.2 | 44.7 |