TABLE 2.
Comparison of Ct values in monoassay and multiplex assay after real-time amplification of a dilution series of an L. pneumophila serogroup 1 standard
| CFU/ml | Ct
|
|||
|---|---|---|---|---|
| Monoassay
|
Multiplex assay
|
|||
| L. pneumophila | Legionella spp. | L. pneumophila | Legionella spp. | |
| 100,000 | 27.2 | 27.7 | 27.4 | 27.8 |
| 10,000 | 31.2 | 30.6 | 31.6 | 32.8 |
| 1,000 | 34.3 | 34.5 | 35.2 | 35.5 |
| 100 | 38.5 | 38.3 | 38.6 | 39.4 |
| 10 | 43.4 | 42.4 | 43.2 | 44.7 |