TABLE 1.
A. pleuropneumoniae reference strains and field isolates used for evaluation of the PCR test
| A. pleuropneumoniae species | Strain designation | No. of strains | No. of strains positive by PCR with:
|
|
|---|---|---|---|---|
| A. pleuropneumoniae- specific amplicon (approx 950 bp) | Serotype-specific amplicon | |||
| Reference isolates | ||||
| Serotype 1 | Shope 4074T | 1 | 1 | 0 |
| Serotype 2 | S 1536 | 1 | 1 | 1 (500)a |
| Serotype 3 | S 1421 | 1 | 1 | 0 |
| Serotype 4 | M62 | 1 | 1 | 0 |
| Serotype 5A | K17 | 1 | 1 | 1 (1,100) |
| Serotype 5B | L20 | 1 | 1 | 1 (1,100) |
| Serotype 6 | Femφ | 1 | 1 | 1 (720) |
| Serotype 7 | WF83 | 1 | 1 | 0 |
| Serotype 8 | 405 | 1 | 1 | 0 |
| Serotype 9 | CVJ 13261 | 1 | 1 | 0 |
| Serotype 10 | D 13039 | 1 | 1 | 0 |
| Serotype 11 | 56153 | 1 | 1 | 0 |
| Serotype 12 | 8329 | 1 | 1 | 0 |
| Serotype 13 (biovar 2) | N-273 | 1 | 1 | 0 |
| Serotype 14 (biovar 2) | 3906 | 1 | 1 | 0 |
| Serotype 15 | HS143 | 1 | 1 | 0 |
| Danish field isolates | ||||
| Serotype 1 | 5(5)b | 5 | 0 | |
| Serotype 2 | 46(23) | 46 | 46 (500) | |
| Serotype 5 | 51(36) | 51 | 51 (1,100) | |
| Serotype 6 | 56(46) | 56 | 56 (720) | |
| Serotype 7 | 5(4) | 5 | 0 | |
| Serotype 8 | 5(2) | 5 | 0 | |
| Serotype 12 | 6(2) | 6 | 0 | |
| Nontypeable strains | 8 | 8 | 5 (500) | |
| 1 (720) | ||||
| (−) | ||||
a
The values in parentheses indicate the approximate amplicon size (in base pairs).
b
The values in parentheses indicate the number of strains cross-reacting by latex agglutination.