TABLE 1.
Distribution of the ermB gene and ribotypes among clinical isolates of C. difficile
| Toxin profile | Isolate no. | Isolation date (mo-yr) | Unit | Patient type | McCoy cytotoxicityf | TcdA gene site (bp)b | PCR ribotypec | PCR result for ermBd | MIC (mg/liter)e
|
|
|---|---|---|---|---|---|---|---|---|---|---|
| CM | EM | |||||||||
| TcdA−B+ | 132 | 1-1999 | Hematology | Child | 10−3 | 700 | A | + | >256 | >256 |
| 202 | 10-1999 | Hematology | Child | 10−1 | 700 | A | + | >256 | >256 | |
| 205 | 11-1999 | Hematology | Child | 10−2 | 700 | A | + | >256 | >256 | |
| 250 | 7-2000 | Hematology | Child | 10−3 | 700 | A | + | >256 | >256 | |
| 254 | 8-2000 | Hematology | Child | 10−2 | 700 | E | + | >256 | >256 | |
| 268 | 11-2000 | Hematology | Child | 10−2 | 700 | A | + | >256 | >256 | |
| 313 | 3-1999 | Internal unit | Child | 10−1 | 700 | A | + | >256 | >256 | |
| 376 | 3-1999 | Internal unit | Child | 10−1 | 700 | A | + | >256 | >256 | |
| 3307 | 12-2000 | Internal unit | Child | 10−2 | 700 | A | + | >256 | >256 | |
| 660 | 5-1999 | Orthopedics | Adult | 10−4 | 700 | A | + | >256 | >256 | |
| 874 | 7-1999 | Orthopedics | Adult | 10−1 | 700 | A | + | >256 | >256 | |
| 1186 | 9-1999 | Orthopedics | Adult | 10−1 | 700 | A | + | >256 | >256 | |
| 1632 | 11-1999 | Orthopedics | Adult | 10−1 | 700 | A | + | >256 | >256 | |
| 1745 | 7-2000 | Orthopedics | Adult | 10−2 | 700 | A | + | >256 | >256 | |
| 3169 | 12-2000 | Orthopedics | Adult | 10−2 | 700 | A | + | >256 | >256 | |
| 114 | 1-2000 | Transplantology | Adult | 10−3 | 700 | A | + | >256 | >256 | |
| 231 | 2-2000 | Transplantology | Adult | 10−1 | 700 | A | + | >256 | >256 | |
| 293 | 2-2000 | Transplantology | Adult | 10−1 | 700 | A | + | >256 | >256 | |
| TcdA+B+ | 2 | 1-1999 | Internal unit | Adult | 10−3 | 2,500 | B | + | >256 | >256 |
| 105 | 1-1999 | Internal unit | Adult | 10−1 | 2,500 | G | + | >256 | >256 | |
| 650 | 5-1999 | Internal unit | Adult | 10−3 | 2,500 | H | + | >256 | >256 | |
| 1405 | 11-1999 | Internal unit | Adult | 10−3 | 2,500 | D | + | >256 | >256 | |
| 711 | 6-1999 | Surgery | Adult | 10−3 | 2,500 | I | + | >256 | >256 | |
| 1166 | 9-1999 | Surgery | Adult | 10−2 | 2,500 | B | + | >256 | >256 | |
| 929 | 8-1999 | Hematology | Child | 10−3 | 2,500 | C | − | 1.5 | 0.125 | |
| 248 | 7-2000 | Hematology | Child | 10−2 | 2,500 | E | − | 0.5 | 0.5 | |
| 277 | 9-2000 | Hematology | Child | 10−3 | 2,500 | F | − | 1.5 | 2.56 | |
| 118 | 1-2000 | Orthopedics | Adult | 10−3 | 2,500 | L | − | 3.0 | 0.094 | |
| 188 | 2-2000 | Surgery | Adult | 10−3 | 2,500 | A | − | 0.016 | >256 | |
| 1140 | 9-1999 | Transplantology | Adult | 10−2 | 2,500 | B | − | 0.023 | 0.023 | |
| 1382 | 10-1999 | Transplantology | Adult | 10−2 | 2,500 | K | − | 0.125 | 0.25 | |
| 28 | 1-2001 | Transplantology | Adult | 10−2 | 2,500 | D | − | 0.5 | 0.5 | |
| 1052 | 11-2000 | Transplantology | Adult | 10−2 | 2,500 | J | − | 2.0 | 2.0 | |
| TcdA−B+a | 2428 | 11-1995 | Surgery | Adult | 10−5 | 700 | A | + | >256 | >256 |
| 2785 | 11-1997 | Internal unit | Child | 10−3 | 700 | A | + | >256 | >256 | |
| 2887 | 12-1997 | Orthopedics | Child | 10−1 | 700 | A | + | >256 | >256 | |
| 399 | 2-1998 | Gastroenterology | Child | 10−5 | 700 | A | + | >256 | >256 | |
| 592 | 2-1998 | Internal unit | Adult | 10−3 | 700 | A | + | >256 | >256 | |
| 1110 | 4-1998 | Hematology | Child | 10−3 | 700 | A | + | >256 | >256 | |
| 2233 | 8-1998 | Neurology | Adult | 10−4 | 700 | A | + | >256 | >256 | |
| 2601 | 9-1998 | Orthopedics | Adult | 10−3 | 700 | A | + | >256 | >256 | |
a
Clinical strains control group.
b
PCR with primer pair NK9-VO11 for detection of the variant of the TcdA gene.
c
PCR ribotyping with primer pair SP1-SP2.
d
PCR with primer pair 2980-2981 for detection of the ermB gene. The fragment is 688 bp.
e
CM, clindamycin; EM, erythromycin.
f
Last dilution of supernatants; demonstrated cytopathic effect.