FIG. 1.

Sensitivity of the ASFV PCR assay using the OIE primer pair or novel PPA-1/2 primer set. DNAs extracted from serial dilutions, in serum, of a suspension of ASFV strain Spain 70 (Spa70) with a titer of 1.6 × 10⁶ UHAD₅₀/ml were employed as templates in the PCR assays under reaction conditions described in the text using OIE primers (left) or novel PPA-1/2 primers (right). M, molecular weight marker VI (Roche Molecular Biochemicals).