FIG. 2.

BsmAI restriction endonuclease analysis of amplification products of different ASFV strains. Lanes 1 and 1′, Brazil 78; 2 and 2′, Haiti 78; 3 and 3′, Spain 70; 4 and 4′, Lisbon 60; 5 and 5′, Cape Verde Islands 97; 6 and 6′, Ivory Coast 99; 7 and 7′, Nigeria 2001. Lanes 1, 2, 3, 4, 5, 6, and 7 are amplification products. Lanes 1′, 2′, 3′, 4′, 5′, 6′, and 7′ are amplification products after digestion with BsmA. Reaction conditions are described in the text. M, molecular weight marker V (Roche Molecular Biochemicals).