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. 2003 Sep;41(9):4107–4112. doi: 10.1128/JCM.41.9.4107-4112.2003

FIG. 2.

FIG. 2.

Agarose gel electrophoresis of PCR products amplified with multiplex primers. (A) The purified chromosomal DNA samples from strains of mutans streptococci (A through H) were used as the templates. Lanes: 1, S. cricetus E49; 2, S. cricetus HS1; 3, S. ratti BHT; 4, S. ratti FA1; 5, S. mutans MT8148; 6, S. mutans Xc; 7, S. sobrinus MT8145; 8, S. sobrinus OMZ176; 9, S. mutans LM7; 10, S. mutans MT703R; 11, S. mutans MT6219; 12, S. mutans OMZ175; 13, S. sobrinus 6715; 14, S. sobrinus OU8; 15, S. downei Mfe28; 16, S. downei S28; M, molecular size markers. The capital letters above the lanes indicate serotypes, which correspond to those referred to in the text with lowercase letters. (B) The chromosomal DNA extracted from colonies on strips of Dentocult SM was used as the template. Lanes: 1 and 8, a reference marker of mixed PCR products from all three serotypes; 2, subject 1; 3, subject 2; 4, subject 3; 5, subject 4; 6, subject 5; 7, subject 6; M, molecular size markers.