TABLE 1.
Primers for respiratory virus RT-PCR assay panel
| Virus (reference) | Primer | Length (nt) | Gene | Location | Polarity | Sequence (5′→3′) | Amplicon size (bp) |
|---|---|---|---|---|---|---|---|
| HRSV (24) | RSVAB-F1-FAM | 23 | F | 1124-1146 | + | AACAGTTTAACATTACCAAGTGA | 380 |
| RSVAB-R1 | 23 | 1481-1503 | − | TCATTGACTTGAGATATTGATGC | |||
| HPIV1 (5) | HPIV1-F1-FAM | 22 | HN | 780-801 | + | CCGGTAATTTCTCATACCTATG | 317 |
| HPIV1-R1 | 21 | 1076-1096 | − | CCTTGGAGCGGAGTTGTTAAG | |||
| HPIV2 (5) | HPIV2-F1-FAM | 22 | HN | 845-866 | + | CCATTTACCTAAGTGATGGAAT | 204 |
| HPIV2-R1 | 22 | 1027-1048 | − | GCCCTGTTGTATTTGGAAGAGA | |||
| HPIV3 (5) | HPIV3-F1-FAM | 22 | HN | 884-905 | + | ACTCCCAAAGTTGATGAAAGAT | 103 |
| HPIV3-R1 | 22 | 965-986 | − | TAAATCTTGTTGTTGAGATTGA | |||
| Influenza A virus (3) | FLUA-F1-FAM | 20 | NS1 | 465-484 | + | CTAAGGGCTTTCACCGAAGA | 192 |
| FLUA-R1 | 20 | 637-656 | − | CCCATTCTCATTACTGCTTC | |||
| Influenza B virus (3) | FLUB-F1-FAM | 21 | NS1 | 746-765 | + | ATGGCCATCGGATCCTCAAC | 241 |
| FLUB-R1 | 20 | 967-986 | − | TGTCAGCTATTATGGAGCTG | |||
| RNA control (27) | BACTIN-F2-FAM | 18 | Human β-actin | −8-26 | + | CCTCGCCTTTGCCGATCC | 626 |
| BACTIN-R2 | 23 | 596-618 | − | GGATCTTCATGAGGTAGTCAGTC |