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Antigenicity of different sucrose gradient fractions measured by μ-capture EIA with peroxidase-conjugated anti-TBEV MAb 1786 as a conjugate. After ultracentrifugation in a sucrose gradient, fractions of 250 μl were separated by upward displacement. Fraction number 1 is from the top, and number 22 is from the bottom. A peak in antigenicity was observed in fractions with 36% (wt/vol) sucrose.
