TABLE 2.
Comparison of results obtained by LiPA and DNA sequencing on 36 MDR-MTC isolates
| LiPA patterna | Mutation(s) identified by DNA sequencing | No. of isolatesb |
|---|---|---|
| R5 (Ser531Leu) | TCG→TTG (Ser531Leu) | 11/11 |
| R2 (Asp516Val) | GAC→GTC (Asp516Val) | 1/4 |
| R4a (His526Tyr) | CAC→TAC (His526Tyr) | 2/0 |
| R4b (His526Asp) | CAC→GAC (His526Asp) | 1/0 |
| ΔS1 | CAA→CCA (Gln513Pro) | 1/0 |
| ΔS2 | ATG→GAC (Met515Leu), ATC→TAC (Asp516Tyr) | 1/0 |
| ΔS4 | CAC→CCC (His526Pro), CAC→CTC (His526Leu) | 1/0 |
| 0/1 | ||
| ΔS5 | Not interpretable | 0/1 |
| Wild type | No mutation | 0/1 |
a
The LiPA assay provided the exact type of mutation in 85% of both Danish and Lithuanian isolates.
b
Values are numbers of isolates from Denmark/numbers of isolates from Lithuania.