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. 1996 Nov 26;93(24):13671–13676. doi: 10.1073/pnas.93.24.13671

Figure 1.

Figure 1

Activation of lacZ reporter gene transcription by AP-1 acting alone and in cooperation with NFAT. (A) Structure of the AP-1 bZIP domain (22), with functional domains denoted, bound to a consensus AP-1 site. (B) NFAT, bound to a polypurine tract in ARRE2, cooperatively recruits AP-1 to the adjacent nonconsensus AP-1 site, thereby activating reporter gene expression. AD denotes the B42 activation domain, which is fused to the basic region of Fos. (C) AP-1-driven reporter assay detects the binding of AP-1 alone to a consensus site in yeast. (D) ARRE2-linked reporter assay detects the reconstitution of the cooperative AP-1/NFAT/ARRE2 complex in yeast. Transcriptional activity refers to units of β-galactosidase activity.