TABLE 1.
Sizes of DNA fragments obtained following PCR-RFLP analysis of 16S rRNA genes of Peptostreptococcus type strains with the restriction enzymes CfoI, HinfI, RsaI, and MnlI
| Bacterial species | Size (bp) of fragment obtaineda
|
|||
|---|---|---|---|---|
| CfoI | HinfI | RsaI | MnlI | |
| P. anaerobius | 700, 430, 340 | 1,500 | 460, 360, 350, 150, 120 | ND |
| P. asaccharolyticus | 870, 430, 220 | 750, 260, 220, 120 | 530, 490, 420, 120 | 420, 350, 270, 180, 130 |
| P. indolicus | 870, 430, 220 | 750, 260, 220, 120 | 530, 490, 420, 120 | 350, 270, 190, 170, 130 |
| P. magnus | 1,100, 430 | 1,100, 380 | 460, 360, 180, 150, 120 | ND |
| P. micros | 1,100, 430 | 520, 390, 240, 200, 180 | 560, 360, 180, 150, 120 | ND |
| P. prevotii | 700, 430, 380 | 470, 380, 250, 220, 150, 125 | 370, 330, 180, 150, 120 | ND |
| P. productus | 940, 430, 200 | 950, 380, 100 | 530, 490, 420, 120 | ND |
a
Only DNA fragments at least 100 bp in size are shown. ND, not determined.