TABLE 2.
Primers used in this study
| PCR | Primer | Sequence | Orientationa | GenBank accession no. | Position | PCR product size (bp) |
|---|---|---|---|---|---|---|
| Multiplex cdt | CDT-s1 | 5′-GAAAGTAAATGGAATATAAATGTCCG | F | U04208 | 1292-1317 | 466 |
| U89305 | 1966-1991 | |||||
| CDT-s2 | 5′-GAAAATAAATGGAACACACATGTCCG | F | U03293 | 1019-1044 | ||
| CDT-as1 | 5′-AAATCACCAAGAATCATCCAGTTA | R | U04208 | 1735-1758 | ||
| U89305 | 2409-2432 | |||||
| CDT-as2 | 5′-AAATCTCCTGCAATCATCCAGTTA | R | U03293 | 1462-1485 | ||
| Multiplex cnf | CNF-s | 5′-TTATATAGTCGTCAAGATGGA | F | U42629 | 1638-1658 | 633 |
| X70670 | 1636-1656 | |||||
| U01097 | 892-912 | |||||
| CNF-as | 5′-CACTAAGCTTTACAATATTGA | R | U42629 | 2251-2271 | ||
| X70670 | 2249-2269 | |||||
| U01097 | 1505-1525 | |||||
| cdt-I | CDT-Is | 5′-CAATAGTCGCCCACAGGA | F | U03293 | 1186-1203 | 411 |
| CDT-IIas | 5′-ATAATCAAGAACACCACCAC | R | U03293 | 1587-1597 | ||
| cdt-II | CDT-IIs | 5′-GAAAGTAAATGGAATATAAATGTCCG | F | U04208 | 1291-1317 | 556 |
| CDT-IIas | 5′-TTTGTGTTGCCGCCGCTGGTGAAA | R | U04208 | 1824-1847 | ||
| cdt-III | CDT-IIIs | 5′-GAAAGTAAATGGAATATAAATGTCCG | F | U89305 | 1966-1991 | 555 |
| CDT-IIIas | 5′-TTTGTGTCGGTGCAGCAGGGAAAA | R | U89305 | 2498-2521 | ||
| cdt-IV | CDT-IVs | 5′-CCTGATGGTTCAGGAGGCTGGTTC | F | U03293 | 1078-1095 | 350 |
| CDT-IVas | 5′-TTGCTCCAGAATCTATACCT | R | AY162217 | 340-360 | ||
| cnf-1 | CNF-1s | 5′-GGGGGAAGTACAGAAGAATTA | F | U42629 | 2700-2720 | 1111 |
| X70670 | 2698-2719 | |||||
| CNF-1as | 5′-TTGCCGTCCACTCTCACCAGT | R | U42629 | 3791-3811 | ||
| X70670 | 3789-3809 | |||||
| cnf-2 | CNF-2s | 5′-TATCATACGGCAGGAGGAAGCACC | F | U01097 | 1942-1965 | 1240 |
| CNF-2as | 5′-GTCACAATAGACAATAATTTTCCG | R | U01097 | 3159-3182 |
a
F, forward; R, reverse.