TABLE 1.
Acid-induced proteins identified by using high-throughput screening strategies
| Organism (conditions) and gene | Predicted protein | Organism (conditions) and gene | Predicted protein | |
|---|---|---|---|---|
| S. oralis (pH 5.2, 2D-PAGE) (248) | 60-kDa chaperonin | |||
| Hsp33 protein (redox-regulated chaperone) | ||||
| Cell division protein DivIV A | ||||
| Superoxide dismutase | ||||
| Formate acetyitransferase 1 | ||||
| Pyruvate oxidase | ||||
| NADP-specific glutamate dehydrogenase | ||||
| Glyceraldehyde-3-phosphate dehydrogenase | ||||
| l-Lactate dehydrogenase | ||||
| Fructose bisphosphate aldolase | ||||
| Acetoin utilization AcuB protein | ||||
| Galactose-6-phosphate isomerase, LacB subunit | ||||
| ATP synthase alpha chain | ||||
| ATP synthase beta chain | ||||
| ATP synthase gamma chain | ||||
| ATP synthase delta chain | ||||
| ATP synthase epsilon chain | ||||
| Formate-tetrahydrofolate ligase | ||||
| Oxygen-insensitive NAD(P)H | ||||
| Nitroreductase/dihydropteridine reductase | ||||
| ABC transporter | ||||
| Hypothetical ABC transporter | ||||
| ATP-binding protein | ||||
| EIIA component of MTL phosphotransferase system | ||||
| Nicotinate phosphoribosyltransferase | ||||
| Adenylate kinase | ||||
| ReoA protein | ||||
| Polyribonucleotide nucleotidyltransferase | ||||
| Polypeptide deformylase | ||||
| Membrane protein precursor | ||||
| Succinyl-diaminopimelate desuccinylase | ||||
| Phosphoprotein phosphatase 2C | ||||
| M. tuberculosis (pH 5.5, microarray) (88) | ||||
| Rv3083 | Probable monooxygenase | |||
| Rv30084 (lipR) | Probable acetyl hydrolase | |||
| Rv3085 | Short-chain ADH | |||
| Rv3086 (adhD) | Zinc-containing ADH | |||
| Rv3087 | Conserved hypothetical protein | |||
| Rv3088 | Conserved hypothetical protein | |||
| Rv3089 (fadD13) | Acyl-CoA synthase | |||
| Rv0467 | Isocitrate lyase | |||
| Rv0468 (icl) | 3-Hydroxyacyl-CoA dehydrogenase | |||
| Rv3614c (fadB2) | Conserved hypothetical protein | |||
| Rv3615c | Conserved hypothetical protein | |||
| Rv3616c | Conserved hypothetical protein | |||
| Rv1130 | Conserved hypothetical protein | |||
| Rv1131 (gltA1) | Citrate synthase 3 | |||
| Rv2428 (ahpC) | Alkyl hydroperoxide reductase | |||
| Rv2429 (ahpD) | Member of AhpC family | |||
| Rv1245C | Putative dehydrogenase | |||
| Rv2007c (fdxA) | Ferredoxin | |||
| Rv1404 | Transcriptional regulator (MarR family) | |||
| MT0196 | Hypothetical protein | |||
| Rv1169c (PE) | PE family protein | |||
| Rv0582 | Hypothetical protein | |||
| Rv2450c (rpfE) | Conserved hypothetical protein (resuscitation-promoting factor) | |||
| L. monocytogenes (pH 3.5 and 5.5, 2D-PAGE) (179) | ATP synthase (subunit B) | |||
| Thoredoxin reductase homologue | ||||
| Chaperonin | ||||
| Sigma H factor | ||||
| Ferric uptake regulator | ||||
| Alcohol dehydrogenase | ||||
| YfiV, similar to transcriptional regulator | ||||
| Similar to transcriptional regulator | ||||
| Transcriptional regulator | ||||
| Similar to GroEL | ||||
| S. mutans (pH 5.2, 2D-PAGE) (249) | DnaK | |||
| GroEL | ||||
| Trigger factor, Ppiase | ||||
| Cell division FtsZ | ||||
| Cell division protein FtsA | ||||
| Superoxide dismutase (Mn) | ||||
| Neutral endopeptidase | ||||
| Glucan-1,6-α-glucosidase | ||||
| Phosphoglucomutase/ phosphomannomutase | ||||
| Enolase | ||||
| NADP-specific glutamate dehydrogenase | ||||
| l-Lactate dehydrogenase | ||||
| Enoyl-(acyl-carrier protein) reductase (NADH) | ||||
| Fructose bisphosphate aldolase | ||||
| Acetoin reductase | ||||
| Metal-dependent hydrolase | ||||
| Lactoylglutathione lyase | ||||
| Phosphoenolpyruvate:protein phosphotransferase | ||||
| Multiple sugar-binding transport protein ATP-binding transport protein MsmK | ||||
| EF-G | ||||
| EF-Tu | ||||
| Small-subunit ribosomal protein S1P | ||||
| EF-Ts | ||||
| DNA-directed RNA polymerase alpha chain | ||||
| Ribosome-recycling factor | ||||
| S. mutans (pH 5.5, differential-display PCR) (40) | ||||
| AP11 | Hippurate hydrolase | |||
| AP-712 | Acetolactate synthase, large subunit | |||
| AP-711 | Pyruvate carboxylase subunit B | |||
| GSP781 | Putative secreted protein (S. mutans) | |||
| AP-785 | Conserved hypothetical protein (Thermotoga maritima) | |||
| AP-185 | Branched-chain amino acid transferase | |||
| AR-186 | Aconitase hydratase | |||
| AR-731 | Isocitrate dehydrogenase | |||
| AR-732 | Citrate synthase |