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. 2003 Sep;10(5):775–779. doi: 10.1128/CDLI.10.5.775-779.2003

FIG. 3.

FIG. 3.

PCR amplification of T. rangeli SubTr-specific sequence. (A) Agarose gel electrophoresis of amplified products with TrF3 and TrRv6 primers. (B) Southern blot of gel A after hybridization with digoxigenin-labeled Trcap oligonucleotide. Lanes: 1, T. rangeli DOG-82; 2, T. rangeli triat 1; 3, T. rangeli triat 2; 4, T. rangeli S. Agustín; 5, T. rangeli Palma, 6, T. rangeli JRM; 7, T. rangeli Alba; 8, T. cruzi CL Brener; 9, T. cruzi G; 10, T. cruzi JMP; 11, T. rangeli Mono; 12, Trypanosoma sp. 13, Trypanosoma saimirii; 14, T. lewisi; 15, T. evansi; 16, L. amazonensis; 17, L. braziliensis; 18, L. chagasi; 19, C. fasciculata; 20, Recombinant TrVA5 (8) (positive control). Arrow indicates the 170-bp specific product.