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Immunoreactivity of the Nfa1 protein with a peroxidase-labeled anti-Nfa1 antibody. Trophozoites of N. fowleri in cultivation were treated with an anti-Nfa1 antibody (A) and normal mouse serum (B). Trophozoites of N. fowleri were stained brown. Trophozoites of N. gruberi (C) did not react with an anti-Nfa1 antibody and did not stain brown. Magnification, ×200.
