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Visualization (using an LDH release assay) of the cytotoxicity of N. fowleri trophozoites against CHO cells. CHO cells were grown in cultures in EMEM medium alone (lane 1, rows A, B, and C), in cocultures with N. fowleri trophozoites (lane 1, rows D, E, and F), and in cocultures with N. fowleri trophozoites and an anti-Nfa1 antibody (lane 2, rows D, E, and F). Other wells represent control groups.
