Figure 2.

Analysis of three different homologous insertion events by the two targeting vectors: TVL444P and TVRecNciI. (A) ES cell DNAs were digested with BamHI, and the filter was probed with probe I (Upper) and probe E (Lower), respectively. Clones 4-1 → 4-9, ES cell clones from TVL444P targeting experiment; Clones R-1 → R-9, ES cell clones from TVRecNciI targeting experiment. (B) Sequence analysis of the mouse GC transcripts from ES cells. cDNA clones, obtained by reverse transcription–PCR, of wild-type and targeted alleles were sequenced with an automated DNA sequencer (Applied Biosystems model 373A). Only mutated amino acids are labeled. Nucleotide mutations are indicated by black dots.