FIG. 6.

N-WASP can localize at podosomes and inhibit their formation. The inducible V12Cdc42 PAE cell line was transfected with GFP-N-WASP (a to f) or GFP-N-WASP-ΔWA (g to i), and induced for 24 h with IPTG. Cells were stained with rhodamine-phalloidin to visualize F-actin (red) (a, c, d, f, g, and i), and GFP identifies transfected cells (green) (b, c, e, f, h, and i). In most cells, expression of GFP-N-WASP (d to f) and GFP-N-WASP-ΔWA (g to i) inhibits podosome formation, and N-WASP localized to podosomes (a to c) in a few cells where expression of N-WASP was low. Bar, 50 μm.