Figure 4.

Western blot analysis of dark-grown rbcS transformants. Total soluble cell protein was extracted from wild-type (lane 1), mutant T60-3 (lane 2), and independent rbcS1 (lanes 3–6) and rbcS2 (lanes 7–10) transformants of T60-3. Each protein sample (30 μg) was fractionated with SDS/PAGE. The proteins were transferred to nitrocellulose and probed with rabbit antibody against tobacco Rubisco holoenzyme. The T60-3 mutant accumulates only a trace of large subunit. LS, large subunit; SS, small subunit.